This medium, which comes in a box of four 12 mL vials, is a modification of the original Human Tubal Fluid (HTF) medium with HEPES formulation.
- Loosens adhesions between blastomeres to facilitate removal.
- Decreases manual manipulation times resulting in less cell damage.
- No adverse effects on embryonic development to blastocyst stage.
- Shown to be a critical factor in successful micromanipulation.
- Can be used in human embryos at the 4–10 cell stage.