Pre Implantation Genetic Screening Kit
- Initial attempts to detect aneuploidy in IVF embryos used FISH screening for a limited subset of chromosomes (5-12 chromosomes only).
- Clinical data from these first attempts showed no benefit to IVF success rates.
- This has changed dramatically since the introduction of advanced 24 chromosome preimplantation genetic screening (PGS).
- PGS now assesses the loss or gain of any whole chromosomes.
- Chromosome-specific PCR products from 120bp-4kb How are the printed: As a pooled library of sequences specific to each chromosome.
- Number of array targets: Over 35 million.
- On average 1.2m sequences per spot Replicate targets on chip: 1 spot per chromosome.
- 8 replicates Microarrays per slide: 4 allowing the testing of 4 samples at a time Ease of analysis: There is a single spot per chromosome Software: Excel Macro.
